Ozone Therapy

The Science of Ozone

Dr. Hughes’ primary rationale for use of injected ozone, alongside its benefits to mitigate pain, is its safe and very potent antimicrobial effects as part of sterile technique.

Ozone is a naturally occurring, highly reactive molecule consisting of three atoms of oxygen. Because ozone is so reactive, it is able to heavily stimulate fibroblastic and chondroblastic activity. This high level of reactivity, combined with the innate safety of oxygen, makes ozone a highly therapeutic molecule.

When ozone comes into contact with body fluids, the resulting reactions form more proteins and red blood cells. This increases oxygen supply in your body.

NIH published this clinical review on ozone therapy:

Ozone therapy has been utilized and extensively studied for many decades altogether. Its effects are proven, consistent and with minimal side effects. Medical O3, used to disinfect and treat disease, has been around for over 150 years. Used to treat infections, wounds and multiple diseases, O3‘s effectiveness has been well-documented. It has been used to disinfect drinking water before the turn of the last century. Ozone was known to treat as many as 114 diseases.[] Ozone therapy has been in use since the 1800s and in 1896 the genius Nikola Tesla patented the first O3 generator in the US, later forming the “Tesla Ozone Company.”[] During the first world war (1914-18) doctors familiar with O3‘s antibacterial properties, and with few other medical resources available to them applied it topically to infected wounds and discovered O3 not only remedied infection, but also had hemodynamic and anti-inflammatory properties.[] In the late 1980s, reports had emerged that German physicians were successfully treating HIV patients with 03-AHT (Autohemotherapy).

Prolozone Injections

While prolozone therapy is similar to prolotherapy in that it also involves regenerative injections, prolozone injections contain less dextrose and therefore typically produce less inflammation. Prolozone also includes the injection of ozone into the tissue, which increases the blood supply and flow of healing nutrients while stimulating the deposition and activity of fibroblasts and chondroblasts.

These cells synthesize the collagen and cartilage that the body uses to repair damaged ligaments and joints. The prolozone injections increase cellular repair activity to strengthen and tighten the injured tissues; thereby stabilizing the area, and removing the cause of the pain.

Ozone IV Therapy

  • Produces antioxidants which inactivates free radicals thereby reducing oxidative stress 
  • Slows down aging at the cellular level
  • Immune system support: Scientists discovered IV ozone works on more than 100 other diseases and illnesses, including HIV, Lymes, and Ebstein Barr Virus
  • Improves blood circulation, as more oxygen travels to your cells, tissues and organs.
  • Antibacterial, antifungal, and antiviral abilities
  • Helps the body detox and repair stem cells


Andreula, C., Simonetti, L., de Santis, F., Agati, R., Ricci, R., & Leonardi, M. (2003). Minimally invasive oxygen-ozone therapy for lumbar disk herniation. AJNR Am J Neuroradiol. 24, 996–1000.

Applied Ozone Systems. Medical ozone oxygen therapy references. Retrieved from http://www.appliedozone.com/references.html.

Bocci, V. (1999). Biological and clinical effects of ozone. Has ozone therapy a future in medicine? British Journal of Biomedical Science. 56(4), 270-279.

Bocci, V. (2011). OZONE A new medical drug. Dordrecht, The Netherlands: Springer.

Boyles, S. (2009). Ozone may help herniated disc pain. WebMD. Retrieved from http://www.webmd.com/back-pain/news/20090309/ozone-may-help-herniated-disc-pain.

D’Erme M, Scarchilli A, Artale A, Pasquali Lasagni, M. (1998). Ozone therapy in lumbar sciatic pain. Raidol Med. 95, 1-2.

Elvis, A. M., & Ekta, J. S. (2011). Ozone therapy: A clinical review. Journal of Natural Science, Biology, and Medicine, 2(1), 66–70. http://doi.org/10.4103/0976-9668.82319.

Ozonotherapy History

Phend, C. (2009). Ozone shots as fffective as surgery for back pain. Medpage Today. Retrieved from http://www.medpagetoday.com/MeetingCoverage/SIR/13206.

Re, L., Mawsouf, M., Menendez, S., Leon, O., Sanchez, G., Hernandez., F. (2008). Ozone therapy: Clinical and basic evidence of its therapeutic potential. Archives of Medical Research. 39(1), 17-26.

Sagai, M., & Bocci, V. (2011). Mechanisms of Action Involved in Ozone Therapy: Is healing induced via a mild oxidative stress? Medical Gas Research, 1, 29. http://doi.org/10.1186/2045-9912-1-29


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