Adult Stem Cells

Mesenchymal stem cells as therapeutics

Mesenchymal stem cells (MSCs) are multipotent cells that are being clinically explored as a new therapeutic for treating a variety of immune-mediated diseases. First heralded as a regenerative therapy for skeletal tissue repair, MSCs have recently been shown to...

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Platelet Rich Plasma

Conservative Management of Joint Pain

Conservative Management of Joint Pain from Megan Haines 1. Conservative Management of Joint Pain John C. Hughes, D.O. Aspen Integrated Medicine 2. Overview: Osteopathic Treatment of Joint Pain  Osteopathic Medicine: DO defined, Principles  DO techniques...

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What To Know Before Getting Stem Cells

Link to: What to know before getting stem cells pdf What to know before getting stem cells Dr. John Hughes, DO Aspen Integrative Medicine July 6th, 2017 Disclaimer: I have no material interest or investment in any stem cell companies or products. I. Introduction to...

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PRP for Osteoarthritis Case Study

Arthritis is a major cause of disability and chronic pain and has a significant economical impact. Osteoarthritis (OA) is a progressive and degenerative form of arthritis that does not discriminate against age. Osteoarthritis is difficult to treat, with most current...

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Prolotherapy

Prolozone / Ozone

Osteopathic Manipulative Treatment

Constraint-Induced Movement Therapy

By. Victor W. Mark, MD, Edward Taub, PhD, Gitendra Uswatte, PhD, Khurram Bashir, MD, MPH, Gary R. Cutter, PhD, Camille C. Bryson, MS, PT, Staci Bishop-McKay, BS, and Mary H. Bowman, OTR/L Impaired mobility is a major cause for reduced quality of life in multiple...

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Adult Stem Cells

Balyasnikova, I. V., Prasol, M. S., Ferguson, S. D., Han, Y., Ahmed, A. U., Gutova, M., … & Aboody, K. S. (2013). Intranasal delivery of mesenchymal stem cells significantly extends survival of irradiated mice with experimental brain tumors. Molecular Therapy.

Cox, C. S., Hetz, R. A., Liao, G. P., Aertker, B. M., Ewing‐Cobbs, L., Juranek, J., … & Dash, P. K. (2016). Treatment of severe adult traumatic brain injury using bone marrow mononuclear cells. Stem Cells.

Danielyan, L., Schäfer, R., von Ameln-Mayerhofer, A., Buadze, M., Geisler, J., Klopfer, T., … & Buniatian, G. H. (2009). Intranasal delivery of cells to the brain. European journal of cell biology88(6), 315-324.

Danielyan, L., Beer-Hammer, S., Stolzing, A., Schäfer, R., Siegel, G., Fabian, C., … & Novakovic, A. (2014). Intranasal delivery of bone marrow-derived mesenchymal stem cells, macrophages, and microglia to the brain in mouse models of Alzheimer’s and Parkinson’s disease. Cell transplantation,23(Supplement 1), S123-S139.

Danielyan, L., Schäfer, R., von Ameln-Mayerhofer, A., Bernhard, F., Verleysdonk, S., Buadze, M., … & Koehle, C. (2011). Therapeutic efficacy of intranasally delivered mesenchymal stem cells in a rat model of Parkinson disease. Rejuvenation research14(1), 3-16.

Fransson, M., Piras, E., Burman, J., Nilsson, B., Essand, M., Lu, B., … & Loskog, A. S. (2012). CAR/FoxP3-engineered T regulatory cells target the CNS and suppress EAE upon intranasal delivery. Journal of neuroinflammation9(1), 112.

Hanson, L. R., & Frey, W. H. (2008). Intranasal delivery bypasses the blood-brain barrier to target therapeutic agents to the central nervous system and treat neurodegenerative disease. BMC neuroscience9(Suppl 3), S5.

Johnson, V. E., Stewart, W., & Smith, D. H. (2010). Traumatic brain injury and amyloid-β pathology: a link to Alzheimer’s disease? Nature Reviews. Neuroscience11(5), 361–370. http://doi.org/10.1038/nrn2808.

Liao, G. P., Harting, M. T., Hetz, R. A., Walker, P. A., Shah, S. K., Corkins, C. J., … & Tsao, K. (2015). Autologous bone marrow mononuclear cells reduce therapeutic intensity for severe traumatic brain injury in childrenPediatric critical care medicine: a journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies16(3), 245.

Ratajczak, M. Z., Zuba-Surma, E. K., Wysoczynski, M., Ratajczak, J., & Kucia, M. (2008). Very small embryonic-like stem cells: characterization, developmental origin, and biological significance. Experimental hematology36(6), 742-751.

Roggendorf, W., Opitz, H., & Schuppan, D. (1988). Altered expression of collagen type VI in brain vessels of patients with chronic hypertensionActa Neuropathologica Acta Neuropathol, 77(1), 55-60.

Sovalat, H., Scrofani, M., Eidenschenk, A., & Hénon, P. (2015). Human very small embryonic-like stem cells are present in normal peripheral blood of young, middle-aged, and aged subjects. Stem cells international2016.

van Velthoven, C. T., Kavelaars, A., van Bel, F., & Heijnen, C. J. (2010). Nasal administration of stem cells: a promising novel route to treat neonatal ischemic brain damagePediatric research68, 419-422.

Wei, N., Yu, S. P., Gu, X., Taylor, T. M., Song, D., Liu, X. F., & Wei, L. (2013). Delayed intranasal delivery of hypoxic-preconditioned bone marrow mesenchymal stem cells enhanced cell homing and therapeutic benefits after ischemic stroke in mice. Cell transplantation,22(6), 977-991.

William, H. F. I., Danielyan, L., & Gleiter, C. H. (2016). U.S. Patent No. 9,445,991. Washington, DC: U.S. Patent and Trademark Office.

Zhang, J., Jiang, R., Liu, L., Watkins, T., Zhang, F., & Dong, J. (2012). Traumatic brain injury-associated coagulopathy. Journal of Neurotrauma, 29(17), 2597-2605. doi: 10.1089/neu.2012.2348

Platelet Rich Plasma

Akeda, K., Imanishi, T., Ohishi, K., Masuda, K., Uchida, A., Sakakibara; T., Kasai, Y., Sudo, A. (2012).Intradiscal Injection of Autologous Platelet-Rich-Plasma for the Treatment of Lumbar Disc Degeneration. Department of Orthopaedic Surgery and Spinal Surgery and Medical Engineering.

Alderman, D. (2015). The New Age of ProlotherapyPractical Pain Management. 

American Academy of Orthopaedic Surgeons. (2014). Platelet-rich plasma treatment more effective than cortisone for severe hip bursitis. ScienceDaily. Retrieved February 24, 2016 from www.sciencedaily.com/releases/2014/03/140314093747.htm

Bonakdar, R. A. & Sukiennik, A. (2016). Integrative Pain Management. New York, NY: Oxford University Press.

Boyette, A. & Shanahan, G. (n.d.). Usage of Platelet Rich Plasma in Foot and Ankle PathologyPodiatry Institute.

DeNoon, D. (2010). Platelet-Rich Plasma Helps Tennis Elbow. Fitness & Exercise. Retrieved from WebMD February 24, 2016. http://www.webmd.com/fitness-exercise/20100310/platelet-rich-plasma-helps-tennis-elbow.

de Vos, R. J., Weir, A., van Schie, H. T. M., Bierma-Zeinstra, S. M. A., Verhaar, J. A. N., Weinans, H., & Tol, J. L. (2010). Platelet-Rich Plasma Injection for Chronic Achilles Tendinopathy. The Journal of the American Medical Association, 303(2), 144-149.

Filardo, G., Kon, E., Della Villa, S., Vincentelli, F., Fornasari, P. M., & Marcacci, M.(2010). Use of platelet-rich plasma for the treatment of refractory jumper’s knee. International Orthopaedics (SICOT), 34, 909–915.

Freitag, J. B., & Barnard, A. (2013). To evaluate the effect of combining photo-activation therapy with platelet-rich plasma injections for the novel treatment of osteoarthritisBMJ case reports2013, bcr2012007463.

Gaweda, K., Tarczynska, M., & Krzyzanowski, W. (2010). Treatment of Achilles Tendinopathy with Platelet-Rich Plasma. International Journal of Sports Medicine.

Hauser, R. (2015). Platelet Rich Plasma Therapy for Knee Osteoarthritis. Retrieved from http://www.caringmedical.com/prolotherapy-news/platelet-rich-plasma-knee-osteoarthritis/.

Hunt, K., Bergeson, A., Coffin, C., Randall, R. (2009). Percutaneous curettage and bone grafting for humeral simple bone cysts. Orthopedics, 32(2), 89.

Kon, E., Filardo, G., Delcogliano, M., Lo Presti, M., Russo, A., Bondi, A., Di Martino, A., Cenacchi, A., Fornasari, P. M., & Marcacci, A., (2008). Platelet-rich plasma: New clinical application A pilot study for treatment of jumper’s kneeInternational Orthopaedics (SICOT) International Orthopaedics, 34(6), 909-915.

Mei-Dan, O., Carmont, M. R. (2011). The role of platelet-rich plasma in rotator cuff repairSports Medicine and Arthroscopy Review,19(3), 244-250.

New York. (2013). Platelet-rich Plasma (PRP) Treatment Shows Potential for Knee Osteoarthritis. Hospital for Special Surgery.

Platelet-Rich Plasma (PRP) Therapy. (n.d.). Retrieved March 11, 2016, from http://www.emoryhealthcare.org/sports-medicine/procedures/prp-therapy.html.

Randelli, P., Arrigoni, P, Ragone, V., Aliprandi, A., Cabitza, P. (2011). Platelet rich plasma in arthroscopic rotator cuff repair: a prospective RCT study, 2-year follow-up. Journal of Shoulder and Elbow Surgery, 20(4), 518-528.

Sánchez, M., Anitua, E., Azofra, J., Aguirre, J. J., Andia, I., (2008). Intra-articular injection of an autologous preparation rich in growth factors for the treatment of knee OA: a retrospective cohort study. Clinical and Experimental Rheumatology.

Shen, L., Yuan, T., Chen, S., Xie, X., & Zhang, C. (2017). The temporal effect of platelet-rich plasma on pain and physical function in the treatment of knee osteoarthritis: systematic review and meta-analysis of randomized controlled trialsJournal of Orthopaedic Surgery and Research12, 16. http://doi.org/10.1186/s13018-017-0521-3

Prolotherapy

Alderman, D. (2015). The New Age of ProlotherapyPractical Pain Management. 

Andreula, C. F., Simonetti, L., de Santis, F., Agati, R., Ricci, R., & Leonardi, M. (2003). Minimally Invasive Oxygen-Ozone Therapy for Lumbar Disk HerniationAJNR American Journal Neuroradiology, 24, 996-1000.

Applied Ozone Systems. Medical ozone oxygen therapy references. Retrieved from http://www.appliedozone.com/references.html.

Bocci, V. (1999). Biological and clinical effects of ozone. Has ozone therapy a future in medicine? British Journal of Biomedical Science. 56(4), 270-279.

Bocci, V. (2011). OZONE A new medical drug. Dordrecht, The Netherlands: Springer.

Boyles, S. (2009). Ozone may help herniated disc pain. WebMD. Retrieved from http://www.webmd.com/back-pain/news/20090309/ozone-may-help-herniated-disc-pain.

D’Erme M, Scarchilli A, Artale A, Pasquali Lasagni, M. (1998). Ozone therapy in lumbar sciatic pain. Raidol Med. 95, 1-2.

Elvis, A. M., & Ekta, J. S. (2011). Ozone therapy: A clinical reviewJournal of Natural Science, Biology, and Medicine2(1), 66–70. http://doi.org/10.4103/0976-9668.82319.

Hashemi M, et al. (2015). The effects of prolotherapy aith hypertonic dextrose versus prolozone (intraarticular ozone) in patients with knee osteoarthritis. Anesth Pain Med. 5(5). Retrieved from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4644302/.

Heckel, A. (n.d.). Prolotherapy claims to treat sprains and pains without surgery or pills. Retrieved March 02, 2016, from http://www.dailycamera.com/ci_21263857/prolotherapy-claims-treat-sprains-and-pains-without-surgery.

Ongley, M., Dorman, T., Klein, R., Eek, B., & Hubert, L. (1987). A new approach to the treatment of chronic low back pain. The Lancet330(8551), 143-146.

Phend, C. (2009). Ozone shots as effective as surgery for back pain. Medpage Today. Retrieved from http://www.medpagetoday.com/MeetingCoverage/SIR/13206.

Prolotherapy for chronic ankle pain. (n.d.). Retrieved March 11, 2016, from http://www.getprolo.com/prolotherapy-chronic-ankle-pain/

Rabago, D., Slattengren, A., & Zgierska, A. (2010). Prolotherapy in Primary Care PracticeProlotherapy in Primary Care Practice. National Institute of Health. 37(1), 65-80.

Re, L., Mawsouf, M., Menendez, S., Leon, O., Sanchez, G., Hernandez., F. (2008). Ozone therapy: Clinical and basic evidence of its therapeutic potentialArchives of Medical Research. 39(1), 17-26.

Sagai, M., & Bocci, V. (2011). Mechanisms of Action Involved in Ozone Therapy: Is healing induced via a mild oxidative stress? Medical Gas Research1, 29. http://doi.org/10.1186/2045-9912-1-29

Work, H. I., & FAQs, P. Prolotherapy for Back Pain Treatment.

Yildirim, C., Akmaz, I., Sahin, O., & Keklikci, K. (2011). Simple calcaneal bone cysts. J Bone Joint Surg Br, 93-B(12), 1626-1631.

Prolozone

Andreula, C. F., Simonetti, L., de Santis, F., Agati, R., Ricci, R., & Leonardi, M. (2003). Minimally Invasive Oxygen-Ozone Therapy for Lumbar Disk HerniationAJNR American Journal Neuroradiology, 24, 996-1000.

Bocci, V., Zanardi, I., & Travagli, V. (2011). Oxygen/ozone as a medical gas mixture. A critical evaluation of the various methods clarifies positive and negative aspects.Medical gas research1(1), 1-9.

Boyles, S. (2009). Ozone may help herniated disc pain. WebMD. Retrieved from http://www.webmd.com/back-pain/news/20090309/ozone-may-help-herniated-disc-pain.

D’Erme M, Scarchilli A, Artale A, Pasquali Lasagni, M. (1998). Ozone therapy in lumbar sciatic pain. Raidol Med. 95, 1-2.

Hashemi M, et al. (2015). The effects of prolotherapy aith hypertonic dextrose versus prolozone (intraarticular ozone) in patients with knee osteoarthritis. Anesth Pain Med. 5(5). Retrieved from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4644302/.

Phend, C. (2009). Ozone shots as fffective as surgery for back pain. Medpage Today. Retrieved from http://www.medpagetoday.com/MeetingCoverage/SIR/13206.

Osteopathic Manipulation

Andersson, G., Lucente, T., Davis, A., Kappler, R., Lipton, J., & Leurgans, S. (1999). A Comparison of Osteopathic Spinal Manipulation with Standard Care for Patients with Low Back PainThe New England Journal of Medicine, 341, 1426-1431.

Bonakdar, R. A. & Sukiennik, A. (2016). Integrative Pain Management. New York, NY: Oxford University Press.

Licciardone, J. C. (2008). The epidemiology and medical management of low back pain during ambulatory medical care visits in the United States.Osteopathic Medicine and Primary Care2(1), 1-17.

Ongley, M., Dorman, T., Klein, R., Eek, B., & Hubert, L. (1987). A new approach to the treatment of chronic low back pain. The Lancet330(8551), 143-146.