1. Innovative Case Studies with Ozone Therapy for Treatment of Anosmia, Degenerative Disc Disease, and Body Odor” Dr. John C. Hughes, D.O. April 12-13, 2012 AAOT Conference (Dallas, Texas)

2. Ozone Therapy for Treatment of Anosmia, Degenerative Disc Disease, and Body Odor”  Ozone Therapy for Anosmia–Glycozone Inhaled  Ozone Therapy for Degenerative Disc Disease US Guided Intradiscal Ozone Plus Nutrients  Ozone Therapy for Body Odor  Conclusion

3. Anosmia: Treated by Ozone  Anosmia- Inability to perceive odor  Due to inflammation of the nasal mucosa (or a destruction of one temporal lobe, rarely)  Inflammation is due to chronic mucosa changes in the paranasal sinus lining and the middle and superior turbinates.

4. Anosmia: Causes  Anosmia: smell may be caused by death of olfactory receptor neurons in the nose or by brain injury  Nasal sprays that cause vasoconstriction of the nasal microcirculation can also be contributory  Nasal polyps due chronic sinusitis or allergies are a factor  Several other causes: Drug side effects, hypothyrodism, head trauma http://en.wikipedia.org/wiki/Anosmia

5. Paranasal Sinuses: Chronic Inflammation Leads to Anosmia http://mysinusinfectionsymptoms.com/paranasal-sinuses/

6. Anosmia: Treated by Ozone  Glycozone: Nikola Tesla created his ozonated oil by bubbling ozone through pure olive oil in the presence of a magnetic field for eight weeks.  By 1904, ozonated olive oil, also known as Glycozone, was in the medical literature. http://www.silvermedicine.org/ozonated-olive-oil.html  Does inhaled ozonated olive oil deliver a safe form of O3 to the sinuses and surrounding tissues?

7. Anosmia: Treated by Ozone  If so, the properties of ozone can be counted on as the following: Improved Microcirculation Decreased Inflammation (comparable to steroids without the side effects) Anti-allergic and Anti-microbial effects (http://www.ajnr.org/content/26/5/988.full) A treatment of Anosmia and Sinusitis!

8. Anosmia: Treated by Glycozone  The Technical: Get Bubblers from Longevity Resources Add Sterile or Filtered Water to one Bubbler Add Organic Olive Oil (plus a few drops peppermint or eucalyptus as desired) to other Bubbler Begin O3 at 10 to 16 ug/cc into bubblers (the one with water is first) with Nasal Canula or Mask attached to second bubbler Breathe in deeply for 10-20 minutes (either sitting or lying down)

9. Anosmia: Treated by Glycozone http://www.frankshospitalworkshop.com/equipment/oxygen_concentrators_equipment.html

10. Intervertebral O3 injections–Italian Study  http://www.ajnr.org/cgi/content/full/24/5/996/F2  (American Journal of Neuroradiology)  From 1999 to 2001, 600 patients aged 20–80 years treated with a single dose of oxygen-ozone therapy. (All done in Italian hospitals).  Three hundred patients (group A, received an intradiscal (4 mL) and periganglionic (8 mL) injection of an oxygen-ozone mixture with an ozone concentration of 27 µg/mL

11. Ozone (O3)-Italian Study  The other 300 patients (group B,) received identical oxygen-ozone injections, followed by a periganglionic injection of corticosteroid (1 mL of Depo-Medrone 40 mg and anesthetic (2 mL of Marcain 0.5%  The L4–5 level was the most frequently treated (61.8%); L1–2, 0.7%; L2–3, 1.2%; L3–4, 8.7%; L5-S1, 27.6%.

12. Puncture at L4-L5 performed under CT guidance.

13. FIG 3. Therapeutic outcome 6 months after oxygen-ozone therapy. Light gray bars indicate group A (n=300); dark gray bars, group B (n=300). Numbers at top of bars are percentages.

14. US guided injection of an Intervertebral Disc MRI-L4-5 disc herniation US L4-5 disc herniation

15. Intradiscal Ozone for Degenerative Disc Disease  If Ozone is so great for Intevertebral Disc Herniations, then why not consider it for Degenerated Discs?  “Oxygen-Ozone Therapy for Degenerative Spine Disease in the Elderly”  Servizio di Neuroradiologia Istituto Clinico Cimi di Brescia; * Servizio di Neuroradiologia Spedali Civili di Brescia; Italy  www.centauro.it Rivista Italiana a’i Ossigeno-Ozonoterapia 5: 25-32, 2006

16. Intradiscal Ozone for Degenerative Disc Disease  Bonetti etal injected 129 elderly patients (65 to 93 years old) with spondyloarthrosis or degenerative discs  Patients were given an initial CT guided intradiscal injection followed by 4 paralumbar injections one week apart  61% of patients received improvement after 1 year with 34 % with a resolution of pain; 27% reported improvement but with mild pain; only 1 patient opted for surgical intervention

17. Lumbar MRI: Do You Want to Treat This 68 yo Male Patient?

18. Intradiscal Ozone for Degenerative Disc Disease  68 yo male with severe back pain due to lumbar disc degeneration and nerve compression  Instead of just ozone, why not add some other growth agents and nutrients to the disc?  And inject the disc multiple times as needed, instead of just doing paralumbar shots?  Let’s do some local at 0.5 % procaine (diluted by sterile water), 1 cc glucosamine, 1cc dextrose (diluted to 15%), trace hyaluronic acid and follow it all by O3 at 20 ug/cc (10 cc/ disc)

19. Intradiscal Ozone for Degenerative Disc Disease  Also, let’s pretreat and posttreat the back with lumbar decompression (at the local chiropractors office)  And finally, let’s skip the CT radiation exposure and just use a clinical ultrasound for everything.  The Results: “John, you’ve saved my back. I’m strong again and can hike or do anything I want. I don’t have any back pain.”

20. US Guided Disc Injections More Testimonial  Another Testimonial from B. Theire with pain from degenerative discs and disc herniation  http://aspenintegrativemedicine.com/testimonials

21. Ozone Therapy for Body Odor  Body Odor in Humans: apocrine sweat glands, which secrete the majority of chemical compounds  Skin flora, members of Corynebacterium, manufacture enzymes called lipases  Lipids in sweat are broken down to create smaller molecules like butyric acid, a smelly compound often used in fish bait  Propionic acid (propanoic acid) created by propionibacteria are also present in sweat glands and create a vinegar-like smell  http://en.wikipedia.org/wiki/Body_odor

22. Ozone Therapy for Body Odor  Butyric Acid is a product of anaerobic fermentation  http://en.wikipedia.org/wiki/Butyric_acid  The triglyceride of butyric acid makes up 3% to 4% of butter.  When butter goes rancid, butyric acid is liberated from the glyceride by hydrolysis, leading to the unpleasant odor  The same kind chemical reaction happens in human armpits, anal-genital areas

23. Ozone Therapy for Body Odor  It is theorized that O3 in the form of glycozone (ozonated olive oil) can prevent or slow the anaerobic fermentation process that creates butyric acid from lipids  Ozonated oil may simply slow the ability of the skin flora to break down the lipids as they are dissolved in the glycozone oil  Finally, glycozone has further antimicrobial effects on the organisms, of course

24. Ozone Therapy for Body Odor  The Technical:  Buy Ozonated Olive Oil: Pure Energy is a good brand  Apply every 1-3 days under your armpits or whenver you desire  Note: The ozonated oil should be solid and be rubbed into the skin or hair wherever applied  Smell yourself or your patient after a few days; If needed, apply again  If motivated, add 20 drops of tea tree oil to the ozonated oil

25. Men Smell Like Cheese; Women Smell Like Onions http://www.dailyglow.com/skin/men-smell-like-cheese-women-smell-like-onions.html http://www.pureenergystore.com/servlet/the- 257/organic-ozonated-extra-virgin/Detail

26. Ozone Therapy for Body Odor Lecture Conclusions: Ozone Helps You Smell Ozone is the Bomb for your Back Ozone Helps You Not Smell  Any Questions?